Journal: eLife

Article Title: H3K9me2 orchestrates inheritance of spatial positioning of peripheral heterochromatin through mitosis

doi: 10.7554/eLife.49278

Figure Lengend Snippet: ( A ) Representative immunofluorescent confocal images of murine C2C12 cells illustrating localization of H3K9me2- and H3K9me2S10p-marked chromatin and Lamin B during different stages of mitosis; DNA visualized with DAPI. Scale bars: 5 μm. ( B ) Magnified images of Interphase and Prophase from panel ( A ) demonstrating detachment of the H3K9me2-chromatin from the nuclear lamina concomitant with H3K9me2S10p phosphorylation; scale bar: 1 μm. ( C ) Representative images of cells progressing through telophase as the layer of peripheral H3K9me2-marked heterochromatin (green) is re-established and nuclear lamina (Lamin B, red) is reassembled; dashed boxes in top panels indicate higher resolution images. Scale bars: 5 μm (top) and 1 μm (bottom panels). ( D ) Magnified images of telophase and daughter cells from panel A demonstrating de-phosphorylated H3K9me2-chromatin (green) assembled at the nuclear lamina (Lamin B, red), while the phosphorylated form (H3K9me2S10p, cyan, enchanced brightness) remains localized in the nuclear interior; scale bar: 1 μm. Dashed lines indicate location of corresponding representative line signal intensity profiles (bottom row).

Article Snippet: Peptide , H3K9me2S10p , EpiCypher , Cat# 12–0093 , IF (1:500).

Techniques: Phospho-proteomics

Journal: eLife

Article Title: H3K9me2 orchestrates inheritance of spatial positioning of peripheral heterochromatin through mitosis

doi: 10.7554/eLife.49278

Figure Lengend Snippet: ( A ) C2C12 cells in prophase stained with H3K9me2S10p antibody (green) preincubated with indicated blocking peptides, counterstained with DAPI (blue). ( B ) Histone peptide array analysis of H3K9me2S10p antibody reactivity with indicated peptides.

Article Snippet: Peptide , H3K9me2S10p , EpiCypher , Cat# 12–0093 , IF (1:500).

Techniques: Staining, Blocking Assay, Peptide Microarray

Journal: eLife

Article Title: H3K9me2 orchestrates inheritance of spatial positioning of peripheral heterochromatin through mitosis

doi: 10.7554/eLife.49278

Figure Lengend Snippet:

Article Snippet: Peptide , H3K9me2S10p , EpiCypher , Cat# 12–0093 , IF (1:500).

Techniques: Peptide Microarray, Modification, Recombinant, Plasmid Preparation, Sequencing, Mutagenesis, Software

Journal: eLife

Article Title: H3K9me2 orchestrates inheritance of spatial positioning of peripheral heterochromatin through mitosis

doi: 10.7554/eLife.49278

Figure Lengend Snippet:

Article Snippet: Peptide , H3S10p , EpiCypher , Cat# 12–0041 , IF (1:500).

Techniques: Peptide Microarray, Modification, Recombinant, Plasmid Preparation, Sequencing, Mutagenesis, Software

Journal: Epigenetics & Chromatin

Article Title: De novo methylation of histone H3K23 by the methyltransferases EHMT1/GLP and EHMT2/G9a

doi: 10.1186/s13072-022-00468-1

Figure Lengend Snippet: Sequence and vendor details for synthetic histone peptides

Article Snippet: H3K18me2 , ARTKQTARKSTGGKAPRK(me2)QLK-biotin , Epicypher; 12–0014 , ELISA/antibody validation.

Techniques: Sequencing, Enzyme-linked Immunosorbent Assay, Biomarker Discovery

Journal: Epigenetics & Chromatin

Article Title: De novo methylation of histone H3K23 by the methyltransferases EHMT1/GLP and EHMT2/G9a

doi: 10.1186/s13072-022-00468-1

Figure Lengend Snippet: Sequence and vendor details for synthetic histone peptides

Article Snippet: H3K18me1 , ARTKQTARKSTGGKAPRK(me1)QLK-biotin , Epicypher; 12–0013 , ELISA/antibody validation.

Techniques: Sequencing, Enzyme-linked Immunosorbent Assay, Biomarker Discovery